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Optimization of Initial Cation Concentrations for L-Lactic Acid Production from Fructose by Lactobacillus pentosus Cells.

Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, Syracuse, NY, 13210, USA. The Center for Biotechnology & Interdisciplinary Studies (CBIS) at Rensselaer Polytechnic Institute, Troy, NY, 12180, USA. California State University, Los Angeles (CSULA), Los Angeles, CA, 90032, USA. Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, 999077, China. Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, Syracuse, NY, 13210, USA. sliu@esf.edu.

Applied biochemistry and biotechnology. 2021;(5):1496-1512
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Abstract

In this study, Box-Behnken design was applied to optimize the initial concentrations of 4 cations for L-lactic acid production from fructose by homologous batch fermentation of Lactobacillus pentosus cells. The optimum initial cation concentrations were obtained as 6.542 mM Mg2+, 3.765 mM Mn2+, 2.397 mM Cu2+, and 3.912 mM Fe2+, respectively. The highest L-lactic acid yield and productivity were obtained as 0.935 ± 0.005 g/g fructose and 1.363 ± 0.021 g/(L × h), respectively, with a maximum biomass concentration of 7.97 ± 0.17 g/L. The effectiveness of the optimization by Box-Behnken design was confirmed based on the small errors between predicted results and experimental results shown as 0.3%, - 0.2%, and - 1.2%, respectively. The quadratic models with high accuracy and reliability can be applied to mathematically forecasted the fermentation performance. After the optimization, the lactic acid yield and productivity were significantly improved by 3.7% and 21.0%, respectively.